Paper Chromatography Method for Identification of Ethylene

The identification of barbiturates by paper chromatography.

B ECAUSE barbiturates are relatively easily available and much used drugs, specimens from suspected cases of poisoning are frequently submitted to the laboratory for routine chemical examination. Since their urinary excretion begins early (because they are quickly absorbed), they can often be identified 1-2 hours after administration. The specimen of choice for examination is therefore the urin...

DEVELOPMENT OF A METHOD FOR THE DETERMINATION OF 4-HYDROXY-3-METHOXYMANDELIC ACID (VANILLYLMANDELIC ACID) IN URINE BY PAPER CHROMATOGRAPHY

We have developed a simple and precise paper chromatographic method for the determination of 4-hydroxy-3-methoxymandelic acid (VMA) in urine. Concentrations of VMA in patients with neuroblastoma were increased in comparison to controls. The linearity was excellent in the concentration range tested. The within-assay coefficient of variation for control and patient urine was less than 2.2%. ...

An Improved Method for Identification of Amino Acids in Descending Paper Chromatography

A change in paper shape is described for use in descending paper chromatography. The "flask-shaped" paper provides greater reliability in the identification of amino acids in unknown mixtures. As many as three samples of unknown and/or known components can be run simultaneously on the same paper and under the same conditions. The method has been used by other investigators for the separation of...

Study of phenylketonuria by paper chromatography

A Simple, Rapid Method for Paper Chromatography of Barbiturates.

Barbiturates are extracted quantitatively into ethyl ether from biologic fluids buffered to pH 6. A measured portion of the ether extract is then impinged to dryness and the residue spotted on Whatman No. 2 paper that has been previously dipped in alkali and dried. The paper is developed by descending chromatography with chloroform saturated with water. The barbiturate may then be located and a...